Sequential extraction of carnosic acid, rosmarinic acid and pigments (carotenoids and chlorophylls) from Rosemary by online supercritical fluid extraction-supercritical fluid chromatography
نویسندگان
چکیده
A high degree of selectivity is required during the plant extraction process in order to obtain extracts enriched specific compounds or avoid unwanted ones. Rosemary well known for its antioxidant (carnosic acid, carnosol and rosmarinic acid). The also contains pigments (i.e. carotenoids, chlorophylls) which may cause a colour problem use extract cosmetic formulations, example. Supercritical fluid considered as selective technique extraction. Due physico-chemical properties supercritical fluids, related pressure, temperature modifier addition, it possible carry out sequential with successive conditions collect different fractions that are rich either bioactive compounds. aim this study was selectively carnosic acid acid) (carotenoids from rosemary using optimisation method carried online coupled chromatography (SFE-SFC) system. Two columns polarities were achieve separation targeted every five minutes Four obtained: first one carotenoids obtained pure CO2 (25°C 20 MPa), second 3% polar (EtOH:water 50/50 v/v), third fraction 10% same fourth chlorophylls 30% ethanol modifier. These four samples then analysed by UHPLC-DAD-ESI-QTOF-HRMS identify other extracted how selected impacted their
منابع مشابه
Supercritical Fluid Extraction+supercritical Fluid Chromatography
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ژورنال
عنوان ژورنال: Journal of Chromatography A
سال: 2021
ISSN: ['1873-3778', '0021-9673']
DOI: https://doi.org/10.1016/j.chroma.2020.461709